MD. Mozammel Hossain Lecturer, Department of Biochemistry & Molecular Biology

Research Interest

NA

Journals Papers

In silico characterization and investigation of putative promoter motifs in Ebolavirus genome


The genus Ebolavirus comprises with clinically significant viral pathogens, namely Bundibugyo ebolavirus, Reston ebolavirus, Sudan ebolavirus, Tai Forest ebolavirus and Zaire ebolavirus causing deadly haemorrhagic fever in human with mortality rate approaching 90% and thus posses a potential global health risk. Ebola infection can be characterized by immune suppression results in disintegration of vascular, coagulation and immune system leading to multi organ failure and shock. The recent Ebola epidemic in Africa is spiraling out of control and no approved therapies/vaccines are available yet for its treatment. The genomes of Ebolavirus are approximately 18.8-19.0 kb long; encode both structural and non structural proteins. The structural proteins play key role in attachment, entry, stability, gene expression and pathogenesis of virus within the host. Therefore, in this in silico approach the complete genome sequences of five different species of Ebolavirus were used to characterize and investigate the putative promoter motifs. The promoter sequences were identified in all the species along with their name, sequence, weight and location within gene by PROSCAN Version 1.7. A grand total of 107 promoters were detected within different Ebolavirus genome; out of which 31, 29, 33, 10 and 4 promoter sequences were found in Bundibugyo ebolavirus, Reston ebolavirus, Sudan ebolavirus, Tai Forest ebolavirus and Zaire ebolavirus respectively. The genome size and G+C (%) of different Ebolavirus were almost equal. This current study may helps in identifying and analyzing various promoter motifs in Ebolavirus, thus understanding their roles in the regulation of gene expression, cell specificity and development. It may also be helpful in designing effective expression vector as well as live attenuated vaccine, hence inventing progressive target specific delivery system and fruitful gene therapy against different Ebolavirus species.


 
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Azospirillum AS BIOFERTILIZER AND BANGLADESH PERSPECTIVE

Azospirillum has potential use as biofertilizer in agriculture. A review of Azospirillum as biofertilizer and Bangladesh perspective has been discussed. Habitat and distribution of Azospirillum have been reviewed. The species of Azospirillum, their isolation, cultivation and preservation have been reviewed. General characteristics and identification of Azospirillum have also been discussed. In this paper, all the possible mode of action of Azospirillum as biofertilizer has also critically reviewed and the crops affected has also been discussed. Studies on Azospirillum carried out in Bangladesh still now, as biofrtilizer also reviewed. Commercial use of Azospirillum has also been discussed in this paper.


 

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Effects of Azospirillum isolates from paddy fields on the growth of rice plants

Inoculation study in pot culture showed that Azospirillum inoculation could significantly increase all the plant growth parameters including germination. To observe the plant growth promoting effects of Azospirillum isolates, ten isolates isolated from paddy fields of North Bengal were used to inoculate “BRRI dhan-28” seeds. Inoculated rice seeds were germinated on petri dish and compared with control. Both inoculated and controlled germinated rice seeds were sown in the soil earthen pots. Some plant growth parameters were considered and compared with control. All of the isolate inoculations increased rice seed germination (%) compared to control. Inoculation with most of the isolates showed that Azospirillum inoculation could significantly increase the growth in terms of height; no. of leaf/plant; length and breadth of leaf; and fresh and dry weight/plant of rice plant.

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Isolation and identification of Azospirillum isolates from different paddy fields of North Bengal


Samples of rhizosphere soil, non-rhizosphere soil and roots of rice plants were collected from the particular locations of the 3 districts (Bogra, Naowgaon and Dinajpur) of North Bengal. Twenty different colonies of Azospirillum spp. were isolated from the different samples from which ten isolates were finally selected for detailed study on the basis of their better growth in Nfb semi-solid medium. The selected isolates were M-1, M-2, M-3, M-4, M-5, M-6, M-7, M-8, M-9 and M-10. The selected isolates were thoroughly characterized on the basis of their response to a number of morphological and physiological tests. Form the confirmation to the identification based on the characteristics of these isolates Bergey’s Manual of Determinative Bacteriology was followed. In this investigation, six selected isolates M-3, M-4, M-6, M-7, M-8 and M-10 were identified as A. lipoferum; three isolates M-1, M-2 and M-5 were identified as A. brasilense and one isolate M-9 was identified as A. halopraeferns. Variations were found in all of the isolates in colonial, morphological and physiological characteristics and in the ability of fermenting various carbohydrates. All of the selected isolates developed characteristics scarlet colonies on RC medium.


 
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Determination of pH, caffeine and reducing sugar in energy drinks available in Bangladesh


Though the popularity of energy drinks is increasing day by day no considerable chemical studies on it has not yet been done in Bangladesh. This investigation was carried out to determine the pH, levels of caffeine and reducing sugar contents in five energy drinks available in local market in Rajshahi, Bangladesh. pH were measured by pH meter. Quantitative estimation of caffeine was performed by a simple and fast standard UV spectrophotometric method, using carbon tetrachloride as the extracting solvent. Reducing sugar content of the energy drink was also determined spectrophotometrically. Results showed that the pH of the beverages were perfectly acidic ranging from 2.85 to 3.11. The minimum caffeine level was observed in Brand-4 (40.34 mg/serving), while Brand-5 showed the highest caffeine content (244.57 mg/serving) showing a range from 40.34 to 244.57 mg/serving. The levels of caffeine in all energy drink samples are well below the maximum allowable limits set by the food regulatory bodies, except Brand-5. The reducing sugar content of the energy drinks ranged from 73.80 mg/serving to 136.08 mg/serving. Brand-1 has the lowest and Brand-4 has the highest reducing sugar providing an idea about the sugar contents of energy drinks. Further investigations are needed to determine the suitability of these energy drinks.


 
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Design and Prediction of Potential RNAi (siRNA) Molecules for 3' UTR PTGS of Different Strains of Zika Virus: A Computational Approach


Zika virus is an aedes mosquito borne pathogen belonging to the member of Flaviviridae subgroup causes an emerging disease called Zika fever, known as a benign infection usually presenting as influenza like illness with cutaneous rash. Nowadays epidemic outbreak caused by Zika virus is highly contagious and incurable with present technologies; thus considered as a major health risk which need enhanced surveillance. Genetic studies on Flavivirus have shown that, the 3' untranslated region (UTR) is consists of seven highly conserved stem loop structure and is important for viral replication and pathogenesis. Therefore, 3' UTR of Zika virus can be utilized as suitable target for controlling Zika virus mediated infection. Viral infection can be reduced by RNA interference (RNAi) technology in which double stranded RNA (siRNA and miRNA) molecules mediate the post transcriptional gene silencing (PTGS) of genes in a sequence specific manner. However genetic variability has been determined in different viral isolates; it is a great challenge to design potential siRNA (small interfering RNA) molecules to repress the expression of respective target gene rather than any other viral gene simultaneously. This work is done using various computational tools to design 21 nucleotides long siRNA sequence on the basis of rational siRNA designing method targeting CDS (coding sequence) of 3' UTR of Zika virus. In this study out of one hundred seventy eight computationally identified siRNAs only four most promising siRNA molecules for gene silencing of 3' UTR of Zika virus were verified using other computer aided tools which might lead to suppress the viral activity. Thus, this approach may provide an insight for chemically synthesized RNA molecules as antiviral agent for Zika virus mediated infection and acts as a foundation stone for an efficient therapeutics at genome level.


 
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Nitrogen fixing efficiency and physiological characteristics of Azospirillum isolates from the paddy fields of North Bengal


The microbial activity of ten selected Azospirillum isolates was measured in terms of the amount of CO2 evolved by the isolates after incubation for 5 days which ranged from 6.88 to19.25 mg. Nitrogen fixing efficiency all of the isolates was determined by microkjeldhal method and the nitrogen fixing efficiency ranged from 10.03 to 13.11 mg N/g substrate. The growth of Azospirillum isolates was significant affected by different physiological factors such as pH, temperature and salinity. Most of the isolates showed optimum growth at pH 6.8, temperature 37°C and in the absence of NaCl. Azospirillum has the potential to be used as a substitute and or supplement of N-fertilizers. Further research is needed to estimate N-supplement potentials of biological nitrogen fixation (BNF) system at farm level.

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